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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene has actually been observed in many tumors, which results in chromosome instability, and thus might add to tumorigenesis. This protein was uncovered to affiliate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a essential part in advertising and marketing cell-cycle development during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, have already been described. Two more splice variants had been claimed but specific nucleotide sequence details is not really nevertheless obtainable.

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Blocking peptides are peptides that bind specifically on the concentrate on antibody and block antibody binding. These peptide generally has the epitope recognized with the antibody.

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This guide outlines further more probable optimization of mobile and immunoassay parameters to be certain the best possible final results are...

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This tutorial outlines even more doable optimization of mobile and immunoassay parameters to guarantee the very best success are...

Antibodies sure to the blocking peptide now not bind to your epitope over the concentrate on protein. By evaluating the staining through the blocked antibody compared to the antibody on your own, one can see which staining is specific.

Concentrate on Information and facts Cyclin E belongs to your remarkably conserved cyclin household, whose associates are characterized by a extraordinary periodicity in protein abundance from the mobile cycle. Cyclins perform as regulators of CDK kinases. Unique cyclins exhibit distinct expression and degradation styles which lead towards the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose action is necessary for cell cycle G1/S transition. This protein accumulates for the G1-S stage boundary and is degraded as cells development via S section.

The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...

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